Table 1. Anti-SARS-CoV2 spike mAbs demonstrate broad neutralization across SARS-CoV-2 spike RBD variants.
Eight anti-spike antibodies were tested against sixteen SARS-CoV-2 spike protein RBD variants identified from viral sequences circulating through end of March 2020. The listed variants were encoded into pVSV-SARS-CoV-2-S (mNeon) pseudoparticles and neutralization assays were performed in Vero cells. IC50(M) values are shown for each variant. There was no observed neutralization with hIgG1 isotype control (N/A).
| Anti-SARS-CoV-2 spike monoclonal antibodies | |||||||||
| Variants | REGN10989 | REGN10987 | REGN10933 | REGN10934 | REGN10964 | REGN10954 | REGN10984 | REGN10986 | Isotype control |
| Wild-type | 7.23 × 10–12 | 4.06 × 10–11 | 4.28 × 10–11 | 5.44 × 10–11 | 5.70 × 10–11 | 9.22 × 10–11 | 9.73 × 10–11 | 9.91 × 10–11 | N/A |
| Q321L | 1.46 × 10–11 | 5.02 × 10–11 | 6.85 × 10–11 | 6.84 × 10–11 | 5.65 × 10–11 | 2.32 × 10–10 | 2.75 × 10–10 | 2.06 × 10–10 | N/A |
| V341I | 1.61 × 10–11 | 3.38 × 10–11 | 3.37 × 10–11 | 7.42 × 10–11 | 1.13 × 10–10 | 2.52 × 10–10 | 2.49 × 10–10 | 1.92 × 10–10 | N/A |
| A348T | 7.33 × 10–12 | 2.98 × 10–11 | 4.13 × 10–11 | 1.42 × 10–10 | 3.52 × 10–11 | 1.84 × 10–10 | 2.01 × 10–10 | 1.03 × 10–10 | N/A |
| N354D | 1.14 × 10–11 | 2.68 × 10–11 | 5.89 × 10–11 | 9.76 × 10–11 | 1.93 × 10–10 | 2.84 × 10–10 | 2.64 × 10–10 | 2.49 × 10–10 | N/A |
| S359N | 4.30 × 10–12 | 2.41 × 10–11 | 2.12 × 10–11 | 3.04 × 10–11 | 6.83 × 10–11 | 1.09 × 10–10 | 1.23 × 10–10 | 8.91 × 10–11 | N/A |
| V367F | 1.33 × 10–11 | 1.78 × 10–11 | 2.40 × 10–11 | 3.20 × 10–11 | 8.92 × 10–11 | 1.29 × 10–10 | 1.53 × 10–10 | 1.49 × 10–10 | N/A |
| K378R | 1.21 × 10–11 | 2.40 × 10–11 | 3.52 × 10–11 | 4.65 × 10–11 | 6.19 × 10–11 | 1.65 × 10–10 | 1.88 × 10–10 | 1.54 × 10–10 | N/A |
| R408I | 1.09 × 10–11 | 1.71 × 10–11 | 1.98 × 10–11 | 2.75 × 10–11 | 4.96 × 10–11 | 9.88 × 10–11 | 1.35 × 10–10 | 6.14 × 10–11 | N/A |
| Q409E | 2.12 × 10–11 | 4.06 × 10–11 | 5.65 × 10–11 | 5.94 × 10–11 | 6.61 × 10–11 | 2.64 × 10–10 | 1.52 × 10–10 | 1.95 × 10–10 | N/A |
| A435S | 1.10 × 10–11 | 3.88 × 10–11 | 4.71 × 10–11 | 8.07 × 10–11 | 7.90 × 10–11 | 2.11 × 10–10 | 2.18 × 10–10 | 1.51 × 10–10 | N/A |
| K458R | 7.51 × 10–12 | 1.68 × 10–11 | 3.43 × 10–11 | 3.46 × 10–11 | 5.46 × 10–11 | 1.45 × 10–10 | 1.59 × 10–10 | 1.00 × 10–10 | N/A |
| I472V | 2.27 × 10–11 | 4.18 × 10–11 | 9.17 × 10–11 | 9.40 × 10–11 | 1.01 × 10–10 | 3.44 × 10–10 | 2.61 × 10–10 | 2.24 × 10–10 | N/A |
| G476S | 6.80 × 10–12 | 1.86 × 10–11 | 1.41 × 10–10 | 3.51 × 10–11 | 3.42 × 10–11 | 1.83 × 10–10 | 2.10 × 10–10 | 1.13 × 10–10 | N/A |
| V483A | 8.78 × 10–12 | 2.60 × 10–11 | 1.54 × 10–11 | 4.43 × 10–11 | 4.50 × 10–11 | 1.12 × 10–10 | 1.71 × 10–10 | 9.70 × 10–11 | N/A |
| Y508H | 1.71 × 10–11 | 2.75 × 10–11 | 4.77 × 10–11 | 6.73 × 10–11 | 1.02 × 10–10 | 2.05 × 10–10 | 2.83 × 10–10 | 2.01 × 10–10 | N/A |
| H519P | 4.51 × 10–12 | 2.20 × 10–11 | 3.03 × 10–11 | 3.56 × 10–11 | 4.45 × 10–11 | 1.40 × 10–10 | 1.08 × 10–10 | 6.14 × 10–11 | N/A |