| Fragmented DNA sample (from step 11) | 75 μl |
| 10× PNK Buffer | 10 μl |
| UltraPureTM Water | 2.4 μl |
| Natural dNTP Mix (25mM) | 1.6 μl |
| T4 DNA Polymerase | 5 μl |
| T4 PNK | 5 μl |
| Klenow DNA Polymerase | 1 μl |
| Total volume = 100 μL | |
| Mix well and spin down. |
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