| 1 cycle: | 30 s 98°C (initial denaturation) |
| 18 cycles: | 10 sec 98°C (denaturation) |
| 30 sec 65°C (annealing) | |
| 30 sec 72°C (extension) | |
| 1 cycle: | 5 min 72°C (final extension) |
| Indefinitely 4°C (hold) |
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