Figure 3.
Copper zinc superoxide dismutase 1 (SOD1) overexpression in vivo abrogates maternal hyperglycemia-induced iNOS expression. Levels of iNOS mRNA ( A) and iNOS protein (B) were determined in E8.75 WT embryos from non-diabetic controls (NC), WT embryos and SOD1 overexpressing embryos from diabetic mellitus (DM) WT mice mated with SOD1-Transgenic (Tg) male mice. SOD1 overexpressing embryos harbor the human SOD1 transgene and SOD1 was detected by a human specific antibody. The upper panel of B was representative images of Western blotting. The graph in the lower panel of B was data of densitometric analysis. * indicates significant difference (p < 0.05) when compared to other groups. In A, n = 6, and in B, n = 3. Experiments were repeated three times with embryos from three different mothers in each group.