Figure 8.
SLITRK1 protein is confined to striosomes and direct pathway neurons in the developing striatum. Fluorescence confocal analysis of P7 mouse brain sections immunolabeled with SLITRK1 (red) and striatal markers (green); cell nuclei are stained with DAPI (blue). A–C: SLITRK1 colocalizes with the μ-opioid receptor, a marker of striosomes. D–I: Considerable overlap was observed between SLITRK1 and both substance P (D–F) and dopamine 1 receptor (G–I), both of which are expressed predominantly in direct pathway neurons. J–L: SLITRK1 has a staining pattern reciprocal to that of enkephalin, a marker of indirect pathway neurons. C,F,I,L are merged images of the preceding panels. A magenta-green copy of this figure is included as Supporting Information Figure 5. Scale bar = 50 µm.