Figure 5.
SLITRK1 is expressed in cortical projection neurons but not in GABAergic interneurons in P7 mouse brain. Fluorescence confocal analysis of cortical sections immunolabeled with SLITRK1 (in red) and appropriate cell-type-specific markers (in green); cell nuclei are stained with DAPI (in blue). A–D: SLITRK1 colocalizes with the following markers: SMI-32, a marker of pyramidal projection neurons (A); BCL11B, a transcription factor found in layer 5 corticospinal and corticotectal neurons (B); and FOXP2, a transcription factor confined to subcortical projection neurons residing predominantly in layer 6 and sparsely in layer 5 (C); partial overlap was also observed with dopamine 1 receptor (D1R; D). E–H: No colocalization was observed between SLITRK1 and various interneuron-specific markers, such as GABA, a marker of GABAergic interneurons (E); or with markers of interneuron subtypes, including NOS1 (F), PVALB (G), and CALB2/calretinin (H). I: SLITRK1 does not colocalize with the astrocytic marker GFAP. Arrows indicate SLITRK1-expressing neurons (red) that also express the indicated cell-type-specific markers. A magenta-green copy of this figure is included as Supporting Information Figure 2. Scale bar = 10 µm.