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. Author manuscript; available in PMC: 2011 Dec 1.
Published in final edited form as: J Invest Dermatol. 2011 Feb 24;131(6):1291–1299. doi: 10.1038/jid.2011.6

Figure 5. Suppression of proliferation by fisetin involves Mitf.

Figure 5

(A) Western blot and RT-PCR analysis of fisetin treated cells showed a dose-dependent inhibitory effect of fisetin on Mitf protein and mRNA levels. Equal loading was confirmed by β-actin. Data shown are representative of at least two independent experiments with similar results (B) Western blot analysis of 451Lu cell transfected with the Mitf expression plasmid pCMV5a-Mitf (1μg) and treated without/with fisetin (60 μM) for 24 h. Equal loading was confirmed by β-actin (C) Flowcytometric analysis of 451Lu cell transfected with the Mitf expression plasmid pCMV5a-Mitf (1μg) and treated without/with fisetin (60 μM) for 24 h. Following FACS analysis, cellular DNA histograms were analyzed by ModiFitLT V3.0. The data are representative of duplicate experiments.