Fig. 1.
Ligands induce phosphorylation and promote binding of PPARγ1 in HT-29 cells. (A) Stimulation with the ligands pioglitazone (Pio), rosiglitazone (Rosi), troglitazone (Tro), ciglitazone (Ci), 15d-PGJ2, and WY-14643 (WY). (B) Western blot and quantifications of phospho-PPARγ relative to total PPARγ with different concentrations of pioglitazone, along with the PPARα-specific ligand WY-14643 (WY) at 2 h of stimulation. (C) Time course of phosphorylation caused by pioglitazone (Pio). (D) Stimulation with GW9662 (GW) and pioglitazone (Pio). All Western blots of nuclear extracts were sequentially probed with phospho-antibody (P-Pγ1) and total PPARγ antibody. All data are representative of at least five independent experiments.