Figure 2.
rml1 Rescue and Phenocopy.
(A) rml1 seedlings at 14 days after germination (DAG) grown on two-fifths MS medium. Note the short (∼1 mm) roots.
(B) rml1 seedlings at 14 DAG grown on two-fifths MS medium supplemented with 250 μM γ-GC.
(C) rml1 seedlings at 14 DAG grown on two-fifths MS medium supplemented with 250 μM GSH.
(D) Top, rml1 seedlings at 7 DAG grown on two-fifths MS medium; middle, wild-type seedlings at 7 DAG grown on two-fifths MS medium supplemented with 2.5 mM BSO, a specific inhibitor of γ-GCS; bottom, wild-type seedlings at 7 DAG grown on two-fifths MS medium. Note the similarity in root growth between the rml1 mutants and the wild-type plants grown on BSO. Wild-type plants develop relatively long roots at the same age when grown on BSO-free medium.
(E) Histograms show the results of a representative experiment in which wild-type and segregating rml1 populations (containing rml1/rml1:RML1/rml1:RML1/RML1 seeds in a 1:2:1 ratio) were germinated on MS medium or medium supplemented with 500 μM γ-GC or 750 μM GSH. The plants were grown vertically for 6 days before the root lengths were measured.
.