Figure 3.
Map-Based Identification of the vte5-1 Mutation.
(A) Low-resolution mapping. Four hundred F3 plants were genotyped to show linkage to the genetic markers MOK16_64764 and MAC12_30751 on chromosome V. cM, centimorgan.
(B) Narrowing the interval between two core markers. Additional markers were assayed to identify a 700-kb region containing the mutation.
(C) High-resolution mapping. An additional 2000 F3 plants were scored using single nucleotide polymorphism markers within the 700-kb region. Markers T32M21_26901 and T32M21_66646 were found to be the closest flanking markers based on the available recombinants, narrowing the interval to 37 kb.
(D) The entire 37-kb region containing seven open reading frames (ORFs) was sequenced from the lt1 mutant.
(E) A single G→A point mutation was detected in exon 5 of T32M21_90. It is predicted to convert a Trp codon to an opal nonsense codon.